Direct detection of molluscum contagiosum virus in clinical specimens by dot blot hybridization
نویسندگان
چکیده
منابع مشابه
Direct detection of molluscum contagiosum virus in clinical specimens by dot blot hybridization.
A dot blot hybridization protocol was developed for the direct detection of molluscum contagiosum virus (MCV) DNA in clinical specimens submitted for virus isolation. Samples were concentrated by high-speed centrifugation and treated with proteinase K; this was followed by a single phenol-chloroform extraction step. The DNA was denatured, and the entire volume was spotted onto a nitrocellulose ...
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A nonradioactive dot blot hybridization assay for human parvovirus B19 DNA was set up by using a biotin-labeled DNA probe and streptavidin-alkaline phosphatase conjugate. The assay was used to examine 4,895 specimens referred for B19 virus diagnosis during 1987. Of 48 specimens that gave positive reactions for B19 DNA, 41 were confirmed virus positive by electron microscopy (n = 36), radioimmun...
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Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
متن کاملMolluscum Contagiosum
a common bath. Not much work has been done from the point of view of the treatment of the disease and the production of immunity so as to prevent epidemics which may be a serious menace in boarding schools. The disease is said to be self-limiting. Though this may be so it still runs a long course ?at least six months?and within that time there is the danger of infecting several people or of sta...
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OBJECTIVE A PCR-reverse dot blot hybridization (RDBH) assay was developed for rapid detection of rpoB gene mutations in 'hot mutation region' of Mycobacterium tuberculosis (M. tuberculosis). METHODS 12 oligonucleotide probes based on the wild-type and mutant genotype rpoB sequences of M. tuberculosis were designed to screen the most frequent wild-type and mutant genotypes for diagnosing RIF r...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1991
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.29.9.1959-1962.1991